Journal: Molecular Medicine

Article Title: Wnt5a-mediated autophagy contributes to the epithelial-mesenchymal transition of human bronchial epithelial cells during asthma

doi: 10.1186/s10020-024-00862-3

Figure Lengend Snippet: Schematic illustration of the mechanisms of Wnt5a-induced EMT in HBECs. This hypothesis illustrates a molecular pathway in HBECs leading to airway remodeling, typically associated with classical autophagy pathway and EMT. In short, Wnt5a induced by HDM or IL-4 leads to the overactivation of autophagy in HBECs through the Ca 2+ /CaMKII signaling pathway, subsequently promoting bronchial epithelial EMT and triggering airway remodeling

Article Snippet: The antibodies used in the present research were as follows: rabbit anti-α-Tubulin antibody (1:2000, Servicebio, Wuhan, China); rabbit anti-α-SMA antibody (1:1000, Cell Signaling Technology, USA); rabbit anti-E-cadherin antibody (1:2000, Cell Signaling Technology); rabbit anti-Beclin1 antibody (1:1500, Cell Signaling Technology); rabbit anti-Collagen I antibody (1:1000, Cell Signaling Technology); rabbit anti-LC3 antibody (1:1500, Cell Signaling Technology); rabbit anti-Wnt5a antibody (1:1000, BOSTER, Wuhan, China); rabbit anti-CaMKII antibody (1:1000, BOSTER); rabbit anti-p-CaMKII antibody (1:1000, Cell Signaling Technology).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Thonningianin A ameliorates acetaminophen-induced liver injury by activating GPX4 and modulating endoplasmic reticulum stress

doi: 10.3389/fphar.2025.1531277

Figure Lengend Snippet: TA mitigates APAP-induced hepatotoxicity through the alleviation of ER stress. (A) Western blotting assessed hepatic GRP78 expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (B) q-PCR was conducted to evaluate the transcript levels of the Grp78 gene. (C) Western blotting assessed hepatic phosphorylated-PERK, phosphorylated-eIF2α, PERK, and eIF2α protein expressions. Band intensities were quantified using ImageJ. (D) Western blotting assessed hepatic ATF4 expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (E) q-PCR was conducted to evaluate the transcript levels of the Atf4 gene. All data were presented as mean ± SD, n = 3 for Western blotting, n = 6 for others. * P < 0.05 vs. corresponding control.

Article Snippet: The following primary antibodies were used: BCL-2 (1:1,000, #ET1702-53), BAX (1:1,000, #ET1603-34), ATF4 (1:1,000, #ET1612-37)—all from HUABIO, China; GRP78 (1:1,000, #AF5366), p-PERK (1:1,000, #DF7576), p-eIF2α (1:1,000, #AF3087), CHOP (1:1000, #AF6277) —all from Affinity Biosciences, China; GPX4 (1:1,000, #BM5231), eIF2α (1:1,000, #M04387-5)—all from Boster, China; PERK (1:1,000, #24390-1-AP, Protenintech, China); β-actin (1:20,000, #AC026, ABclonal, China).

Techniques: Western Blot, Expressing, Control

Journal: Frontiers in Pharmacology

Article Title: Thonningianin A ameliorates acetaminophen-induced liver injury by activating GPX4 and modulating endoplasmic reticulum stress

doi: 10.3389/fphar.2025.1531277

Figure Lengend Snippet: Liver-specific GPX4 knockdown inhibits TA-regulated ER stress in APAP-induced hepatotoxicity. (A) Western blotting assessed hepatic GRP78 expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (B) q-PCR was conducted to evaluate the transcript levels of the Grp78 gene. (C) Western blotting assessed hepatic phosphorylated-PERK, phosphorylated-eIF2α, PERK, and eIF2α protein expressions. Band intensities were quantified using ImageJ. (D) Western blotting assessed hepatic ATF4 expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (E) q-PCR was conducted to evaluate the transcript levels of the Atf4 gene. All data were presented as mean ± SD, n = 3 for Western blotting, n = 5 for others. * P < 0.05 vs. corresponding control.

Article Snippet: The following primary antibodies were used: BCL-2 (1:1,000, #ET1702-53), BAX (1:1,000, #ET1603-34), ATF4 (1:1,000, #ET1612-37)—all from HUABIO, China; GRP78 (1:1,000, #AF5366), p-PERK (1:1,000, #DF7576), p-eIF2α (1:1,000, #AF3087), CHOP (1:1000, #AF6277) —all from Affinity Biosciences, China; GPX4 (1:1,000, #BM5231), eIF2α (1:1,000, #M04387-5)—all from Boster, China; PERK (1:1,000, #24390-1-AP, Protenintech, China); β-actin (1:20,000, #AC026, ABclonal, China).

Techniques: Knockdown, Western Blot, Expressing, Control

Journal: Molecules

Article Title: The Large Molecular Weight Polysaccharide from Wild Cordyceps and Its Antitumor Activity on H22 Tumor-Bearing Mice

doi: 10.3390/molecules28083351

Figure Lengend Snippet: WCP promotes Cyto-c/Caspase8/3 and inhibits IL-10/STAT3/Bcl2 pathway. ( A – F ) Relative mRNA expression of IL-6, IL-Iβ, NF-κB, TNF-α, Bax, and Bcl2. * p < 0.05 compared to model group.

Article Snippet: Rabbit monoclonal antibodies against Caspase8 (PTM-6085) antibody were purchased from PTM Biolab Co., Ltd. (Hangzhou, China), and signal transducer and activator of transcription3 (Stat3, BM4052), phosphorylated signal transducer and activator of transcription3 (p-STAT3 Y705, BM4835) were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing

Journal: Molecules

Article Title: The Large Molecular Weight Polysaccharide from Wild Cordyceps and Its Antitumor Activity on H22 Tumor-Bearing Mice

doi: 10.3390/molecules28083351

Figure Lengend Snippet: WCP promotes Cyto-c/Caspase8/3 and inhibits IL-10/STAT3/Bcl2pathway. ( A , F ) Photographs of the proteins in each group. ( B – E , G – I ) Relative protein expression of Cyto-c, Caspase8, Caspase3, p-STAT3, Bcl2, Bax, and Bax/Bcl2. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to model group.

Article Snippet: Rabbit monoclonal antibodies against Caspase8 (PTM-6085) antibody were purchased from PTM Biolab Co., Ltd. (Hangzhou, China), and signal transducer and activator of transcription3 (Stat3, BM4052), phosphorylated signal transducer and activator of transcription3 (p-STAT3 Y705, BM4835) were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing

Journal: Experimental Biology and Medicine

Article Title: Liraglutide prevents fast weight gain and β-cell dysfunction in male catch-up growth rats

doi: 10.1177/1535370214567614

Figure Lengend Snippet: Effects of HFD catch-up growth and liraglutide treatment on β-cell function and apoptosis. Pdx-1 protein (a1) and mRNA (a2) levels of islets at the end of week 8. Pdx-1 protein levels of islets were assessed by Western blot and the results were expressed as the ratios of Pdx-1 and GAPDH. The Pdx-1 mRNA levels were detected by qPCR and expressed as the ratios of Pdx-1 and β-actin. Protein levels of Bcl-2 (b1) and Caspase-3 p 12 subunit (c1) were detected by Western blot and the results were expressed as the ratios of Caspase-3 p12 subunit or Bcl-2 with GAPDH. The mRNA levels of Bcl-2 (b2) and Procaspase-3 (c2) were expressed as the ratios of Proaspase-3 or Bcl-2 with β-actin. All the results are expressed as mean ± SEM. *p < 0.01 versus the NC group; #p < 0.01 versus the CUG group; &p < 0.01 versus the CUGL group

Article Snippet: Primer sequences Western blot analysis Protein extracts from islets (20 μg from each rat) were subjected to Western blot analysis using mouse antipancreatic duodenal homeobox-1 (Pdx-1) monoclonal antibody (1:800; Cell Signaling Technology, Danvers, MA, USA), mouse anti-Caspase-3 p12 subunit monoclonal antibody (1:500, Boster Bio-Engineering, China), mouse anti-B-cell lymphoma (Bcl-2) polyclonal antibody (1:800, Bioworld, USA), and mouse anti-GAPDH antibody (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Cell Function Assay, Western Blot